Journal: International Journal of Molecular Sciences

Article Title: In Vitro Cytotoxicity of D18 and Y6 as Potential Organic Photovoltaic Materials for Retinal Prostheses

doi: 10.3390/ijms23158666

Figure Lengend Snippet: Functional assessment of mouse ex-vivo retina by electrophysiology via microelectrode array (MEA) recording. ( A ) Ex vivo retina sample placed on a glass MEA coated with D18/Y6. The white dashed rectangle indicated the window of the exposed MEA surface to ensure contact between the tissue and the electrodes to enable recording. Scale bar: 400 µm. ( B ) Firing rate during a continuous 1 h recording. The plot shows the average firing rate (FR) values for retinal ganglion cells in 3 different retina samples and the average of the 3 samples (black line—mean). Small gray line indicates FRs on single electrodes, colored areas indicate the standard deviation of the mean FR. ( C ) Characteristic photoreceptor-degenerated retina rhythmic activity (retinal waves) recorded from the same electrode at the start (t = 0′) and the end (t = 60′) of the one-hour continuous recording.

Article Snippet: Recordings were performed using a conventional glass MEA (59 recording electrodes, 30 μm diameter, 200 μm spacing, Multi Channel Systems MCS GmbH) coated with D18/Y6.

Techniques: Functional Assay, Ex Vivo, Microelectrode Array, Standard Deviation, Activity Assay

Journal: Korean Circulation Journal

Article Title: Lower Loading Dose of Prasugrel Compared with Conventional Loading Doses of Clopidogrel and Prasugrel in Korean Patients Undergoing Elective Coronary Angiography: A Randomized Controlled Study Evaluating Pharmacodynamic Efficacy

doi: 10.4070/kcj.2014.44.6.386

Figure Lengend Snippet: Platelet reactivity at baseline and peak platelet inhibition. Platelet reactivity measured at baseline (A, B, and C) and at the time of peak platelet inhibition (D, E, and F) using LTA, VerifyNow, and MEA. The baseline platelet reactivity values were statistically identical between study groups, whereas platelet reactivity at the time of peak platelet inhibition exhibited significant differences. Although platelet reactivity values for the clopidogrel 600 mg group were significantly higher than both prasugrel groups, there was no statistical difference between the prasugrel 30 mg and 60 mg groups. LTA: light transmission aggregometry, MEA: multiple electrode aggregometry.

Article Snippet: Platelet reactivity was measured using three different methods; light transmission aggregometry (LTA), the VerifyNow assay (Accumetrics, San Diego, CA, USA), and multiple electrode aggregometry (MEA, Dynabyte Medical, Munich, Germany).

Techniques: Inhibition, Transmission Assay

Journal: Korean Circulation Journal

Article Title: Lower Loading Dose of Prasugrel Compared with Conventional Loading Doses of Clopidogrel and Prasugrel in Korean Patients Undergoing Elective Coronary Angiography: A Randomized Controlled Study Evaluating Pharmacodynamic Efficacy

doi: 10.4070/kcj.2014.44.6.386

Figure Lengend Snippet: Comparison of inhibition (%) between study groups. Percent inhibition was significantly lower in the clopidogrel 600 mg group than for both prasugrel groups. However, there was no statistical difference between the prasugrel groups. LTA: light transmission aggregometry, MEA: multiple electrode aggregometry.

Article Snippet: Platelet reactivity was measured using three different methods; light transmission aggregometry (LTA), the VerifyNow assay (Accumetrics, San Diego, CA, USA), and multiple electrode aggregometry (MEA, Dynabyte Medical, Munich, Germany).

Techniques: Inhibition, Transmission Assay

Journal: Cell Death & Disease

Article Title: Tau P301L disengages from the proteosome core complex and neurogranin coincident with enhanced neuronal network excitability

doi: 10.1038/s41419-024-06815-2

Figure Lengend Snippet: All experiments were undertaken in DIV12 cultures cultured in CytoView micro-electrode array (MEA) multi-well plates using the Maestro-Pro MEA system (Axion Biosystems). A Each well contains 16 electrodes that can record local activity B Fluorescent microscopy shows DIV12 primary cortical neurons transduced with AAV-Syn1-eGFP and cultured on a micro-electrode plate. Scale bar = 275 µm. C An example of a raster plot, which shows firing over time, taken at baseline from untransduced neurons. Each row corresponds to an individual electrode. Action potentials are recorded as spikes (black lines) and this is termed ‘firing’. A burst (blue) occurs when multiples action potentials fire in rapid succession in the locality of an electrode; a minimum threshold is set at 50 spikes within 100 ms. A network burst was classified as at least 35% of electrodes recording bursts simultaneously; this shows that the neurons throughout the culture are connected. D Representative raster plots showing 60 s recordings of untransduced neurons at baseline and following treatment with 0.625 µM 4AP/12.5 µM Bicuculline (Bic). Baseline recordings show spontaneous firing and bursting. Treatment with 4AP/Bic stimulates synchronous and oscillatory firing across the well, as shown by a switch to network bursting. E 4AP/Bic treatment significantly increases activity and ( F ) burst strength ( t -test, **** P < 0.0001, n = 3 technical repeats). G Representative raster plots comparing neurons overexpressing eGFP, eGFP-Tau WT or eGFP-Tau P301L at baseline and following treatment with 4AP/Bic. H At baseline, the activity and ( I ) burst strength of neurons overexpressing eGFP-Tau P301L is significantly greater than those expressing eGFP or eGFP-Tau WT . Following treatment with 4AP/Bic, activity but not burst strength is significantly greater with eGFP-Tau P301L than eGFP or eGFP-Tau WT (two-way ANOVA with Tukey’s multiple comparisons test, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, n = 4 technical repeats).

Article Snippet: All experiments were undertaken in DIV12 cultures cultured in CytoView micro-electrode array (MEA) multi-well plates using the Maestro-Pro MEA system (Axion Biosystems).

Techniques: Cell Culture, Activity Assay, Microscopy, Transduction, Expressing

Journal: Scientific Reports

Article Title: Effects of fibrillin mutations on the behavior of heart muscle cells in Marfan syndrome

doi: 10.1038/s41598-020-73802-w

Figure Lengend Snippet: MEA measurements of the extracellular field potentials for the control line (H9), the corrected CMs and the MFS CMs after 20–25 days after start of cardiac differentiation. ( A ) The baseline measurements without the addition of isoproterenol (ISO). The extracellular field potentials are shown for 10 nM ( B ) and 100 nM ISO ( C ). The bar plots show the differences between control, corrected and MFS CMs for RR interval ( D ), SDNN ( E ), SDSD ( F ) and coefficient of variance ( G ). Corrected CMs (n = 9, black), MFS CMs (n = 8, red). Poincaré plot from three representative replicates of the control (blue), corrected (black) and MFS CMs (red) ( H ). The three clusters of MFS are magnified in the right corner of figure ( H ). The level of significance is indicated by asterisks: p values less than 0.001 are indicated with three asterisks and p values less than 0.01 indicated with two asterisks.

Article Snippet: The established in vitro cardiac model for MFS was studied by means of multi electrode array (MEA), cyclic strain imparted with the Flexcell, atomic force microscopy (AFM) and video analysis, revealing abnormalities in the behavior of MFS CMs.

Techniques: Control

Journal: Scientific Reports

Article Title: Effects of fibrillin mutations on the behavior of heart muscle cells in Marfan syndrome

doi: 10.1038/s41598-020-73802-w

Figure Lengend Snippet: MEA measurements of control, corrected and MFS CMs after treatment with serial isoproterenol (ISO) concentrations of 5, 10, 25, 50 and 100 nM. Bar plots show differences between the three lines at each ISO concentration for RR interval ( A ), SDNN ( B ), SDSD ( C ) and the coefficient of variance presented as factor of the baseline ( D ). The Spearman’s rank correlation is indicated in panel A. Corrected CMs (n = 6, black), MFS CMs (n = 7, red), H9 control (n = 5, blue).

Article Snippet: The established in vitro cardiac model for MFS was studied by means of multi electrode array (MEA), cyclic strain imparted with the Flexcell, atomic force microscopy (AFM) and video analysis, revealing abnormalities in the behavior of MFS CMs.

Techniques: Control, Concentration Assay